Journal: Frontiers in Immunology

Article Title: CD68 as a multi-omic prognostic biomarker in digestive system cancers: correlations with tumor-infiltrating immune cells and immune checkpoints

doi: 10.3389/fimmu.2025.1599677

Figure Lengend Snippet: Boxplots of CD68 gene expression in human DSC compared with paired normal tissues by using gene array data. (A) , CD68 expression in COAD (n=41paired), ** p= 0.0044. (B) , CD68 expression in ESCA (n=8paired), ns, p =0.107. (C) , CD68 expression in LIHC (n=80paired), ns, p =0.619. (D) , CD68 expression in PAAD (n=4paired), ns, p =0.1, (E) , CD68 expression in STAD (n=27paired),* p =0.0138. The comparison of CD68 expression in pared tumor and adjacent normal tissues was used by running a paired Wilcoxon statistical test.

Article Snippet: The rehydrated sections were incubated overnight at 4°C with the rabbit anti-human CD68 polyclonal antibody (1:50, ABclonal, No.: A13286, USA).

Techniques: Gene Expression, Expressing, Comparison

Journal: Frontiers in Immunology

Article Title: CD68 as a multi-omic prognostic biomarker in digestive system cancers: correlations with tumor-infiltrating immune cells and immune checkpoints

doi: 10.3389/fimmu.2025.1599677

Figure Lengend Snippet: The protein expression of CD68 in human DSC tissues compared with normal tissues. (A) The protein expression of CD68 was significantly higher in COAD (n=24paired) than in its adjacent normal tissues(*). (B) The protein expression of CD68 was lower in ESCA (n=24paired) than in its adjacent normal tissues (ns). (C) The protein expression of CD68 was lower in LIHC (n=24paired) than in its adjacent normal tissues (ns). (D) The protein expression of CD68 was lower in PAAD (n=24paired) than in its adjacent normal tissues (ns). (E) The protein expression of CD68 was significantly lower in STAD (n=24paired) than in its adjacent normal tissues(*). The quantification of IHC was formalized into mean optical density detected by Image-Pro Plus2.0. Bars show the means ± SD. Difference was statistically significant (*p < 0.05). Original magnification: 200×. Scale bar: 100 μm.

Article Snippet: The rehydrated sections were incubated overnight at 4°C with the rabbit anti-human CD68 polyclonal antibody (1:50, ABclonal, No.: A13286, USA).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: CD68 as a multi-omic prognostic biomarker in digestive system cancers: correlations with tumor-infiltrating immune cells and immune checkpoints

doi: 10.3389/fimmu.2025.1599677

Figure Lengend Snippet: Kaplan-Meier survival curves comparing the high and low protein expression of CD68 in COAD and STAD. (A) OS of COAD (n=1336), (B) OS of STAD (n=875). Red curve indicates patients with high expression of CD68, while black curve indicates patients with low expression of CD68. HRs with 95%CIs and log-rank P-values were calculated and shown in each curve. P < 0.05 was considered statistically significant.

Article Snippet: The rehydrated sections were incubated overnight at 4°C with the rabbit anti-human CD68 polyclonal antibody (1:50, ABclonal, No.: A13286, USA).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: CD68 as a multi-omic prognostic biomarker in digestive system cancers: correlations with tumor-infiltrating immune cells and immune checkpoints

doi: 10.3389/fimmu.2025.1599677

Figure Lengend Snippet: Correlation of CD68 expression with six TIICs in DSC. (A) CD68 expression has significant negative relation with tumor purity and B cell, significant positive correlation with tumor-infiltrating levels of CD4+ T cell, CD8+ T cell, macrophage, myeloid dendritic cell and cancer associated fibroblast in COAD. (B) CD68 expression has significant negative relation with tumor purity and significant positive correlation with tumor-infiltrating levels of B cell, CD4+ T cell, CD8+ T cell, macrophage, myeloid dendritic cell and has no relation with cancer associated fibroblast in ESCA. (C) CD68 expression has significant negative relation with tumor purity and significant positive correlation with tumor-infiltrating levels of B cell, CD4+ T cell, CD8+ T cell, macrophage, myeloid dendritic cell and cancer associated fibroblast in LIHC. (D) CD68 expression has significant negative relation with tumor purity and significant positive correlation with tumor-infiltrating levels of CD8+ T cell, macrophage, myeloid dendritic cell and cancer associated fibroblast, has no relation with B cell, CD4+ T cell in PAAD. (E) CD68 expression has significant negative relation with tumor purity, significant positive correlation with tumor-infiltrating levels of B cell, CD4+ T cell, CD8+ T cell, macrophage, myeloid dendritic cell and cancer associated fibroblast in STAD. Each dot represents a single tumor sample. P < 0.05 is considered as significant.

Article Snippet: The rehydrated sections were incubated overnight at 4°C with the rabbit anti-human CD68 polyclonal antibody (1:50, ABclonal, No.: A13286, USA).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: CD68 as a multi-omic prognostic biomarker in digestive system cancers: correlations with tumor-infiltrating immune cells and immune checkpoints

doi: 10.3389/fimmu.2025.1599677

Figure Lengend Snippet: Correlation of CD68 expression with four common TICs (PDCD1, CTLA4, IDO1 and CD40) in DSC. (A) CD68 expression has significant positive correlation with PDCD1 in COAD (n=458), ESCA (n=185), LIHC (n=371), PAAD (n=179) and STAD (n=415). (B) CD68 expression has significant positive correlation with CTLA4 in COAD (n=458), ESCA (n=185), LIHC (n=371), PAAD (n=179) and STAD (n=415). (C) CD68 expression has significant positive correlation with IDO1 in COAD (n=458), ESCA (n=185), LIHC (n=371), PAAD (n=179) and STAD (n=415). (D) CD68 expression has significant positive correlation with CD40 in COAD (n=458), ESCA (n=185), LIHC (n=371), PAAD (n=179) and STAD (n=415). P < 0.05 is considered as significant.

Article Snippet: The rehydrated sections were incubated overnight at 4°C with the rabbit anti-human CD68 polyclonal antibody (1:50, ABclonal, No.: A13286, USA).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: CD68 as a multi-omic prognostic biomarker in digestive system cancers: correlations with tumor-infiltrating immune cells and immune checkpoints

doi: 10.3389/fimmu.2025.1599677

Figure Lengend Snippet: mIF Analysis of CD68+ Macrophage Polarization and PD-L1 Co-localization in COAD and STAD. (A) mIF images of COAD samples: Composite image (Merge) of all markers; DAPI (blue) +CD68 (pink); DAPI (blue)+CD163 (green); DAPI (blue)+iNOS (red); DAPI (blue)+PD-L1 (yellow). (B) mIF images of STAD samples: Composite image (Merge) of all markers; DAPI (blue)+CD68 (pink); DAPI (blue)+CD163 (green); DAPI (blue)+iNOS (red); DAPI (blue)+PD-L1 (yellow). Original magnification: 40×. Scale bars: 25μm.

Article Snippet: The rehydrated sections were incubated overnight at 4°C with the rabbit anti-human CD68 polyclonal antibody (1:50, ABclonal, No.: A13286, USA).

Techniques: